Produce isolates of the Escherichia coli Ont:H52 serotype that carry both Shiga toxin 1 and stable toxin genes

Steven R Monday; Christina Keys; Patricia Hanson; Yuelian Shen; Thomas S Whittam; Peter Feng

doi:10.1128/AEM.72.4.3062-3065.2006

Produce isolates of the Escherichia coli Ont:H52 serotype that carry both Shiga toxin 1 and stable toxin genes

Appl Environ Microbiol. 2006 Apr;72(4):3062-5. doi: 10.1128/AEM.72.4.3062-3065.2006.

Authors

Steven R Monday¹, Christina Keys, Patricia Hanson, Yuelian Shen, Thomas S Whittam, Peter Feng

Affiliation

¹ Division of Microbiological Studies, US Food and Drug Administration, HFS-516, 5100 Paint Branch Parkway, College Park, MD 20740, USA.

Abstract

Produce isolates of the Escherichia coli Ont:H52 serotype carried Shiga toxin 1 and stable toxin genes but only expressed Stx1. These strains had pulsed-field gel electrophoresis profiles that were 90% homologous to clinical Ont:H52 strains that had identical phenotypes and genotypes. All Ont:H52 strains had identical single nucleotide polymorphism profiles that are suggestive of a unique clonal group.

MeSH terms

Bacterial Toxins / genetics*
Bacterial Toxins / metabolism
Bacterial Typing Techniques
Escherichia coli / classification
Escherichia coli / genetics
Escherichia coli / isolation & purification*
Escherichia coli / pathogenicity
Escherichia coli Proteins / genetics
Escherichia coli Proteins / metabolism
Hemolysin Proteins
Serotyping
Shiga Toxin 1 / genetics*
Shiga Toxin 1 / metabolism
Vegetables / microbiology*

Substances

Bacterial Toxins
Escherichia coli Proteins
Hemolysin Proteins
Shiga Toxin 1
enterohemolysin