Abstract
The most common human brain tumours - gliomas - have poor prognosis with and without treatment. The current therapy conditions act sub-lethally and cannot effectively suppress the proliferation of glioma cells. Here we show differential protein expression patterns in surviving human malignant U87-MG glioma cells under clinically relevant chemo/radiotherapy. In parallel experiments, the cells underwent either irradiation (2 Gy, 200 KV X-ray) or chemotreatment with 30 microg/mL of temozolomide in the cultivation medium or combined chemo/radiation treatment. The cell cultures were treated during 5 days from day 4 until day 9 of growth. Modulated expression patterns of vimentin and RhoA GTPase indicate a potentially increasing grade of malignancy in treated cell fractions correlating well with extremely aggressive tumour phenotypes observed clinically at recidivation of treated malignant gliomas.
MeSH terms
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Antineoplastic Agents, Alkylating / pharmacology
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Cell Line, Tumor
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Cells, Cultured
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Combined Modality Therapy
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DNA-Binding Proteins / drug effects
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism
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Dacarbazine / analogs & derivatives
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Dacarbazine / pharmacology
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Gene Expression Regulation, Neoplastic / drug effects*
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Glioma / drug therapy*
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Glioma / metabolism
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Glioma / radiotherapy*
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Humans
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Intracellular Signaling Peptides and Proteins / drug effects
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Intracellular Signaling Peptides and Proteins / genetics
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Intracellular Signaling Peptides and Proteins / metabolism
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Perilipin-3
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Pregnancy Proteins / drug effects
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Pregnancy Proteins / genetics
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Pregnancy Proteins / metabolism
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Proteomics*
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Temozolomide
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Vesicular Transport Proteins
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rhoA GTP-Binding Protein / drug effects
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rhoA GTP-Binding Protein / genetics
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rhoA GTP-Binding Protein / metabolism
Substances
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Antineoplastic Agents, Alkylating
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DNA-Binding Proteins
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Intracellular Signaling Peptides and Proteins
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PLIN3 protein, human
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Perilipin-3
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Pregnancy Proteins
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Vesicular Transport Proteins
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Dacarbazine
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rhoA GTP-Binding Protein
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Temozolomide