Increased ammonium in culture medium reduces the development of human embryos to the blastocyst stage

Fertil Steril. 2006 Feb;85(2):526-8. doi: 10.1016/j.fertnstert.2005.10.018.

Abstract

The objective of this prospective study was to evaluate the effect of ammonium accumulated in sequential media and determined by enzymatic spectrophotometric method on the blastocyst development in 281 human embryos from 100 stimulated and natural in vitro fertilization (IVF) cycles. Ammonium concentration was increased in 62% of cycles and was correlated negatively with the blastocyst development after classical IVF, but not after intracytoplasmic sperm injection (ICSI).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blastocyst / drug effects*
  • Culture Media / chemistry*
  • Embryo, Mammalian / physiology*
  • Embryonic Development / drug effects
  • Female
  • Fertilization in Vitro*
  • Humans
  • Male
  • Osmolar Concentration
  • Prospective Studies
  • Quaternary Ammonium Compounds / analysis*
  • Quaternary Ammonium Compounds / pharmacology*
  • Spectrophotometry
  • Sperm Injections, Intracytoplasmic

Substances

  • Culture Media
  • Quaternary Ammonium Compounds