New caerin antibiotic peptides from the skin secretion of the Dainty Green Tree Frog Litoria gracilenta. Identification using positive and negative ion electrospray mass spectrometry

Micheal J Maclean; Craig S Brinkworth; Daniel Bilusich; John H Bowie; Jason R Doyle; Lyndon E Llewellyn; Michael J Tyler

doi:10.1016/j.toxicon.2006.01.019

New caerin antibiotic peptides from the skin secretion of the Dainty Green Tree Frog Litoria gracilenta. Identification using positive and negative ion electrospray mass spectrometry

Toxicon. 2006 May;47(6):664-75. doi: 10.1016/j.toxicon.2006.01.019. Epub 2006 Mar 22.

Authors

Micheal J Maclean¹, Craig S Brinkworth, Daniel Bilusich, John H Bowie, Jason R Doyle, Lyndon E Llewellyn, Michael J Tyler

Affiliation

¹ Department of Chemistry, The University of Adelaide, Adelaide, SA 5005, Australia.

PMID: 16554081
DOI: 10.1016/j.toxicon.2006.01.019

Abstract

The skin secretion of the Dainty Green Tree Frog Litoria gracilenta contains 16 peptides, which protect the animal from predators, both large and small. A combination of negative and positive ion electrospray mass spectrometry together with Lys-C enzymic digest and Edman sequencing identifies three new wide-spectrum caerin 1 antibiotics, namely Caerin 1.17 [GLFSVLGSVAKHLLPHVAPIIAEKL-NH2], Caerin 1.18 [GLFSVLGSVAKHLLPHVVPVIAEKL-NH2], and Caerin 1.19 [GLFKVLGSVAKHLLPHVAPIIAEKL-NH2], and a narrow spectrum antibiotic Caerin 3.5 [GLWEKVKEKANELVSGIVEGVK-NH2].

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Amphibian Proteins / chemistry*
Animals
Anti-Infective Agents / analysis*
Antimicrobial Cationic Peptides / chemistry*
Anura*
Chromatography, High Pressure Liquid
Molecular Sequence Data
Skin / metabolism*
Spectrometry, Mass, Electrospray Ionization / methods*

Substances

Amphibian Proteins
Anti-Infective Agents
Antimicrobial Cationic Peptides