Abstract
By making use of the gam(+)-plasmid, the so-called gam-dependent radioresistance was studied. This resistance is the result of the interaction between Gam protein (encoded by the gam gene of lambda) and RecBCD enzyme of Escherichia coli. gam-dependent radioresistance is observed in recB+ recC+ recD+ but not in recB+ recC+ recD- cells. It is suggested that Gam protein interacts specifically with the RecD subunit of RecBCD enzyme; the RecBC complex probably retains its activity in the presence of this viral protein.
MeSH terms
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Bacteriophage lambda / genetics*
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Bacteriophage lambda / radiation effects
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DNA Helicases / genetics*
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DNA Helicases / radiation effects
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DNA-Binding Proteins
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Escherichia coli / enzymology
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Escherichia coli / genetics
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Escherichia coli / radiation effects*
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Escherichia coli Proteins*
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Exodeoxyribonuclease V
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Exodeoxyribonucleases / genetics*
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Exodeoxyribonucleases / radiation effects
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Gamma Rays
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Plasmids
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Viral Proteins / genetics*
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Viral Proteins / radiation effects
Substances
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DNA-Binding Proteins
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Escherichia coli Proteins
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Viral Proteins
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gam protein, Coliphage
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Exodeoxyribonucleases
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Exodeoxyribonuclease V
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exodeoxyribonuclease V, E coli
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DNA Helicases