Improving catalytic properties of P450 BM3 haem domain electrodes by molecular Lego

Chem Commun (Camb). 2006 Mar 28:(12):1289-91. doi: 10.1039/b517472d. Epub 2006 Feb 13.

Abstract

In this work the catalytic properties of a cytochrome P450 immobilised onto an electrode surface are improved by means of the molecular Lego approach.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / chemistry*
  • Bacterial Proteins / genetics
  • Catalysis
  • Catalytic Domain*
  • Cytochrome P-450 Enzyme System / chemistry*
  • Cytochrome P-450 Enzyme System / genetics
  • Electrochemistry
  • Electrodes
  • Flavodoxin / genetics
  • Mixed Function Oxygenases / chemistry*
  • Mixed Function Oxygenases / genetics
  • Models, Molecular
  • NADPH-Ferrihemoprotein Reductase
  • Polyethylenes / chemistry
  • Protein Engineering*
  • Quaternary Ammonium Compounds / chemistry
  • Recombinant Fusion Proteins / chemistry*
  • Recombinant Fusion Proteins / genetics
  • Spectrophotometry, Ultraviolet
  • Substrate Specificity

Substances

  • Bacterial Proteins
  • Flavodoxin
  • Polyethylenes
  • Quaternary Ammonium Compounds
  • Recombinant Fusion Proteins
  • poly-N,N-dimethyl-N,N-diallylammonium chloride
  • Cytochrome P-450 Enzyme System
  • Mixed Function Oxygenases
  • NADPH-Ferrihemoprotein Reductase
  • flavocytochrome P450 BM3 monoxygenases