Construction of Lactose-Utilizing Xanthomonas campestris with a Mini-Mu Derivative

Appl Environ Microbiol. 1995 Feb;61(2):811-2. doi: 10.1128/aem.61.2.811-812.1995.

Abstract

Xanthomonas campestris is not able to grow in lactose media. The lactose operon from Escherichia coli as part of a mini-Mu phage was integrated at random sites in the chromosome of this bacterium. Clones expressing (beta)-galactosidase were selected. The resulting strain X. campestris 204, is suitable for production of xanthan gum directly from lactose.