Abstract
The crystallization and preliminary X-ray diffraction analysis of the chitin-binding domain of chitinase from a hyperthermophilic archaeon, Pyrococcus furiosus, are reported. The recombinant protein was prepared using an Escherichia coli overexpression system and was crystallized by the hanging-drop vapour-diffusion method. An X-ray diffraction data set was collected to 1.70 A resolution. The crystal belonged to space group P4(3)2(1)2 or P4(1)2(1)2. The unit-cell parameters were determined to be a = b = 48.8, c = 85.0 A.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Chitin / chemistry
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Chitin / metabolism
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Chitinases / chemistry*
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Chitinases / metabolism
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Crystallization
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Escherichia coli / genetics
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Escherichia coli / metabolism
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Protein Binding
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Protein Structure, Tertiary
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Pyrococcus furiosus / enzymology*
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Pyrococcus furiosus / genetics
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Pyrococcus furiosus / metabolism
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Recombinant Proteins / chemistry
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
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X-Ray Diffraction / methods*
Substances
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Recombinant Proteins
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Chitin
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Chitinases