Determination of nucleic acids based on the quenching effect on resonance light scattering of the Y(III)-1,6-bi(1'-phenyl-3'-methyl-5'-pyrazolone-4'-)hexane-dione system

Luminescence. 2006 May-Jun;21(3):129-34. doi: 10.1002/bio.896.

Abstract

Nucleic acids can quench resonance light scattering (RLS) intensity of the Y(III)-1,6-bi(1'-phenyl-3'-methyl-5'-pyrazolone-4'-)hexane-dione(BPMPHD) complex in the pH range 5.0-5.8. Under optimal conditions, there are linear relationships between the quenching of RLS and the concentration of nucleic acids in the range 6.3 x 10(-8)-2.1 x 10(-5) g/mL for fish sperm DNA (fsDNA), 1.2 x 10(-8)-5.0 x 10(-5) g/mL for calf thymus DNA (ctDNA) and 6.0 x 10(-8)-2.0 x 10(-5) g/mL for yeast RNA (yRNA). The detection limits (3 s) of fsDNA, ctDNA and yRNA are 0.7 ng/mL, 3.8 ng/mL and 4.2 ng/mL, respectively.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aldehydes / chemistry*
  • Animals
  • Cattle
  • DNA / analysis
  • DNA / chemistry
  • Fishes
  • Hydrogen-Ion Concentration
  • Male
  • Nucleic Acids / analysis*
  • Nucleic Acids / chemistry*
  • Pyrazoles / chemistry*
  • RNA / analysis
  • RNA / chemistry
  • Scattering, Radiation*
  • Sensitivity and Specificity
  • Spermatozoa / metabolism
  • Thymus Gland / metabolism
  • Yeasts / metabolism

Substances

  • 1,6-bi(1'-phenyl-3'-methyl-5'-pyrazolon-4'-)hexanedione
  • Aldehydes
  • Nucleic Acids
  • Pyrazoles
  • RNA
  • DNA
  • calf thymus DNA