Ultrasonic imaging of biofilms utilizing echoes from the biofilm/air interface

Andrew K Holmes; Johanna Laybourn-Parry; Jacqueline D Parry; Marion E Unwin; Richard E Challis

doi:10.1109/tuffc.2006.1588404

Ultrasonic imaging of biofilms utilizing echoes from the biofilm/air interface

IEEE Trans Ultrason Ferroelectr Freq Control. 2006 Jan;53(1):185-92. doi: 10.1109/tuffc.2006.1588404.

Authors

Andrew K Holmes¹, Johanna Laybourn-Parry, Jacqueline D Parry, Marion E Unwin, Richard E Challis

Affiliation

¹ School of Electrical and Electronic Engineering, University of Nottingham, Nottingham NG7 2RD, UK. eezakh@gwmail.nottingham.ac.uk

PMID: 16471445
DOI: 10.1109/tuffc.2006.1588404

Abstract

Ultrasonic imaging of biofilms in water is difficult due to the very low contrast in acoustic impedance between the biofilm and water. In this paper, biofilms exposed to moist air are scanned through the substrate in order to obtain echoes from the biofilm/air interface. A 50 MHz scanning system was used to scan 1 mm x 1 mm areas of biofilms in a 10 microm grid pattern. Two fast Fourier transform (FFT) based methods for enhancement of the film thickness measurement resolution are compared. Using these techniques, the surface topography of biofilms with thickness less than the acoustic wavelength can be imaged.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Air
Algorithms
Bacteria / isolation & purification*
Biofilms*
Colony Count, Microbial / methods*
Image Interpretation, Computer-Assisted / methods*
Ultrasonography / methods*
Ultrasonography, Doppler, Pulsed / methods*