Abstract
We describe a novel metallothionein gene from Tetrahymena thermophila that has a strong copper-inducible promoter. This promoter can be turned on and off rapidly, making it a useful system for induction of ectopic gene expression in Tetrahymena and enhancing its applications in cell and molecular biology, as well as biotechnology.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Copper / pharmacology*
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Gene Expression Regulation / drug effects*
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Metallothionein / genetics*
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Molecular Sequence Data
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Promoter Regions, Genetic / genetics*
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Recombinant Proteins
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Tetrahymena thermophila / cytology
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Tetrahymena thermophila / drug effects
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Tetrahymena thermophila / genetics*
Substances
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RNA, Messenger
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Recombinant Proteins
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Copper
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Metallothionein