Immunoquantitation of cytochrome C in cardiac perfusate

F Naro; A Fazzini; G Citro; F Malatesta; G Antonini; P Sarti; M Brunori; F Franconi; A Giotti

doi:10.1080/01971529108055070

Immunoquantitation of cytochrome C in cardiac perfusate

J Immunoassay. 1991;12(2):251-62. doi: 10.1080/01971529108055070.

Authors

F Naro¹, A Fazzini, G Citro, F Malatesta, G Antonini, P Sarti, M Brunori, F Franconi, A Giotti

Affiliation

¹ Dept. of Biochemical Sciences Univ. of Rome La Sapienza.

PMID: 1646220
DOI: 10.1080/01971529108055070

Abstract

An indirect three step ELISA has been assessed in order to detect the possible release of cytochrome c, a mitochondrial protein, from isolated and perfused guinea-pig heart. The ELISA described in this study is sufficiently sensitive and accurate to measure extracellular cytochrome c.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cytochrome c Group / analysis*
Cytochrome c Group / metabolism
Cytochrome c Group / standards
Enzyme-Linked Immunosorbent Assay / methods*
Enzyme-Linked Immunosorbent Assay / standards
Evaluation Studies as Topic
Guinea Pigs
Heart / physiology*
In Vitro Techniques
Male
Perfusion
Reference Standards
Sensitivity and Specificity

Substances

Cytochrome c Group