Isolation of Rickettsia felis in the mosquito cell line C6/36

Appl Environ Microbiol. 2006 Feb;72(2):1705-7. doi: 10.1128/AEM.72.2.1705-1707.2006.

Abstract

We report the isolation and establishment of Rickettsia felis in the C6/36 cell line. Rickettsial growth was intense, always with 90 to 100% of cells being infected after few weeks. The rickettsial isolate was confirmed by testing infected cells by PCR and sequencing fragments of three major Rickettsia genes (gltA, ompB, and the 17-kDa protein gene).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aedes / microbiology*
  • Animals
  • Bacterial Outer Membrane Proteins / genetics
  • Bacteriological Techniques
  • Cell Line
  • Genes, Bacterial
  • Rickettsia felis / genetics
  • Rickettsia felis / growth & development
  • Rickettsia felis / isolation & purification*

Substances

  • Bacterial Outer Membrane Proteins