Abstract
We report the isolation and establishment of Rickettsia felis in the C6/36 cell line. Rickettsial growth was intense, always with 90 to 100% of cells being infected after few weeks. The rickettsial isolate was confirmed by testing infected cells by PCR and sequencing fragments of three major Rickettsia genes (gltA, ompB, and the 17-kDa protein gene).
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aedes / microbiology*
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Animals
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Bacterial Outer Membrane Proteins / genetics
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Bacteriological Techniques
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Cell Line
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Genes, Bacterial
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Rickettsia felis / genetics
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Rickettsia felis / growth & development
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Rickettsia felis / isolation & purification*
Substances
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Bacterial Outer Membrane Proteins