Abstract
From the MeOH extract of the flowers of Chrysanthemum sinense, a new flavone glucoside, acacetin 7- O-(3- O-acetyl- beta- D-glucopyranoside), has been isolated together with 27 known compounds including flavonoids, caffeoylquinic acid derivatives, phenolics, and a monoterpenoid glucoside. Their structures were elucidated on the basis of spectroscopic data. Compounds and displayed significant xanthine oxidase inhibitory activity in a concentration-dependent manner, and compounds and showed more potent inhibitory activity, with IC50 values ranging from 0.13 to 2.31 microM, than that of a positive control allopurinol (IC50=2.50 microM). The kinetic study indicated that and displayed competitive-type inhibition like that of allopurinol, while displayed a mixed-type inhibition.
MeSH terms
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Chrysanthemum / chemistry*
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Flavonoids / chemistry
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Flavonoids / isolation & purification*
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Flavonoids / pharmacology
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Flowers / chemistry*
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Glucosides / chemistry
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Glucosides / isolation & purification*
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Glucosides / pharmacology
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Glycosides / chemistry
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Glycosides / isolation & purification
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Glycosides / pharmacology
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Inhibitory Concentration 50
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Molecular Structure
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Monoterpenes / chemistry
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Monoterpenes / isolation & purification
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Monoterpenes / pharmacology
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Phenols / chemistry
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Phenols / isolation & purification
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Phenols / pharmacology
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Quinic Acid / analogs & derivatives
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Quinic Acid / chemistry
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Quinic Acid / pharmacology
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Xanthine Oxidase / antagonists & inhibitors*
Substances
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Flavonoids
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Glucosides
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Glycosides
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Monoterpenes
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Phenols
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acacetin-7-O-glucoside
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caffeoylquinic acid
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Quinic Acid
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Xanthine Oxidase