The paper deals with the development of the PCR-test system that allows the greatest possible number of human adenovirus (AdV) serotypes in the clinical samples to be tested by polymerase chain reaction. Primers have been selected on the basis of analysis of 3'-end sequences of hexon genes of 48 AdV serotypes. Five laboratory human AdV strains of different serotypes and some other DNA viruses were used to show the high sensitivity and specificity of AdV DNA detection. The proposed test system was not inferior to the Russian available analogues in sensitivity and specificity.