Size characterization of green fluorescent protein inclusion bodies in E. coli using asymmetrical flow field-flow fractionation-multi-angle light scattering

J Chromatogr A. 2006 Jul 7;1120(1-2):158-64. doi: 10.1016/j.chroma.2005.11.048. Epub 2006 Jan 18.

Abstract

The goal of this study was to investigate the applicability of asymmetrical flow field-flow fractionation-multi-angle light scattering (AsFlFFF-MALS) for size analysis of green fluorescent protein inclusion bodies (GFPIBs). The size distributions of GFPIBs prepared by various culture conditions were determined. For GFPIBs prepared at 37 degrees C the peak maximum hydrodynamic diameter (d(H)) first increased and then decreased with the increase of the induction times in the presence of 0.1 and 2 mM isopropyl-beta-D-thiogalactoside (IPTG). For GFPIBs prepared at 30 degrees C the peak maximum d(H) was constant at about 700 nm irrespectively of the induction times and IPTG concentrations.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Dose-Response Relationship, Drug
  • Escherichia coli / chemistry
  • Escherichia coli / genetics
  • Escherichia coli / metabolism*
  • Fractionation, Field Flow / instrumentation
  • Fractionation, Field Flow / methods*
  • Green Fluorescent Proteins / analysis*
  • Green Fluorescent Proteins / genetics
  • Green Fluorescent Proteins / metabolism
  • Inclusion Bodies / chemistry*
  • Inclusion Bodies / drug effects
  • Inclusion Bodies / metabolism
  • Isopropyl Thiogalactoside / pharmacology
  • Light
  • Molecular Weight
  • Scattering, Radiation*
  • Time Factors

Substances

  • Green Fluorescent Proteins
  • Isopropyl Thiogalactoside