Abstract
This study reports the drug resistance and clonal relationship of 24 Staphylococcus aureus community acquired isolates from patients attending Maputo Central Hospital, Mozambique, during one year (2002-2003). All the isolates produced beta-lactamase, six strains were resistant to tetracycline alone, three were resistant to erythromycin alone and one was resistant to trimethoprim-sulfamethoxazole; 11 were susceptible to all other drugs tested. Only one strain showed a multiple resistance pattern, including methicillin resistance. To investigate the clonal relationships we applied the ERIC AP-PCR and the SmaI PFGE RFLP methods. Overlapping drug resistances with these two molecular profiles, no significant correlation was obtained. The emergence of methicillin resistance in a multiple resistant strain is of great concern for resistance spreading surveillance in Mozambique.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-Bacterial Agents / pharmacology
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Community-Acquired Infections / microbiology*
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DNA Fingerprinting
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DNA, Bacterial / genetics
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DNA, Bacterial / metabolism
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Deoxyribonucleases, Type II Site-Specific
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Drug Resistance, Multiple, Bacterial
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Erythromycin / pharmacology
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Humans
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Methicillin / pharmacology
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Methicillin Resistance*
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Molecular Epidemiology
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Mozambique
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Polymerase Chain Reaction
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Polymorphism, Restriction Fragment Length
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Staphylococcal Infections / microbiology*
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Staphylococcus aureus / drug effects*
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Staphylococcus aureus / isolation & purification
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Sulfamethoxazole / pharmacology
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Tetracycline Resistance
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Trimethoprim Resistance
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beta-Lactamases / biosynthesis
Substances
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Anti-Bacterial Agents
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DNA, Bacterial
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Erythromycin
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CCCGGG-specific type II deoxyribonucleases
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Deoxyribonucleases, Type II Site-Specific
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beta-Lactamases
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Sulfamethoxazole
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Methicillin