1JNH values show that N1...N3 hydrogen bonds are stronger in dsRNA A:U than dsDNA A:T base pairs

J Am Chem Soc. 2005 Dec 28;127(51):17974-5. doi: 10.1021/ja055826l.

Abstract

Here, we show that 1JNH values are on average 0.4 Hz less negative for double-stranded RNA A:U than for DNA A:T base pairs, which, according to existing theory, suggests that RNA N1...N3 hydrogen bond distances are about 0.02 A shorter than those of DNA. Also, there is a statistically relevant trend between 1JNH and 2hDelta13C2 values, which supports the original hypothesis that 2hDelta13C2 values are also sensitive to hydrogen bond distances. Finally, a context dependence is observed for these values, which suggests that hydrogen-bonding and base-stacking interactions are coupled.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Adenine / chemistry
  • Adenine / metabolism
  • DNA / chemistry*
  • DNA / metabolism
  • Hydrogen Bonding
  • Nuclear Magnetic Resonance, Biomolecular / methods
  • RNA, Double-Stranded / chemistry*
  • RNA, Double-Stranded / metabolism
  • Thymidine / chemistry
  • Thymidine / metabolism
  • Uridine / chemistry
  • Uridine / metabolism

Substances

  • RNA, Double-Stranded
  • DNA
  • Adenine
  • Thymidine
  • Uridine