Oxidant and carbonyl stress-related apoptosis in end-stage kidney disease: impact of membrane flux

Blood Purif. 2006;24(1):149-56. doi: 10.1159/000089452.

Abstract

Apoptosis is a highly regulated process which mostly affects cell-mediated immunity. In this open-label, randomized, prospective clinical study, we determined the impact in 10 hemodialysis patients treated with high-, medium-, and low-flux membranes on spontaneous or plasma-induced apoptosis, on monocytes, as well as on oxidant and carbonyl stress. High- and medium-flux membranes significantly reduced patients' plasma-dependent proapoptotic activity on U937 monocytic cell lines. Patients who had the highest levels of plasma-induced proapoptotic activity exhibited the highest plasma levels of advanced oxidation protein products (AOPPs) and carbonyls. Plasma carbonyl residues but not AOPPs were significantly lowered. Finally, a significant correlation could be drawn between the extent of plasma-induced proapoptotic activity and both plasma carbonyl and AOPP levels.

Publication types

  • Randomized Controlled Trial

MeSH terms

  • Adult
  • Apoptosis*
  • Blood Proteins / analysis
  • Female
  • Humans
  • Kidney Failure, Chronic / blood
  • Kidney Failure, Chronic / therapy*
  • Male
  • Membranes, Artificial*
  • Middle Aged
  • Monocytes*
  • Oxidants / blood
  • Oxidation-Reduction
  • Renal Dialysis*
  • U937 Cells

Substances

  • Blood Proteins
  • Membranes, Artificial
  • Oxidants