Immobilization induces alterations in the outer membrane protein pattern of Yersinia ruckeri

J Proteome Res. 2005 Nov-Dec;4(6):1988-98. doi: 10.1021/pr050165c.

Abstract

We compared the outer membrane protein (OMP) pattern of 2-day-old immobilized Yersinia ruckericells (IC) with that of early (FC24) and late (FC48) stationary-phase planktonic counterparts. Fifty-five OMPs were identified. Principal component analysis discriminated between the protein maps of FC and IC. Some OMPs involved in bacterial adaptation were accumulated by both FC48 and IC but the expression of other proteins was controlled by the sessile mode of growth.

MeSH terms

  • Bacterial Outer Membrane Proteins / chemistry*
  • Bacterial Proteins / chemistry
  • Biofilms
  • Cell Culture Techniques / methods
  • Chromatography, Liquid
  • Electrophoresis, Gel, Two-Dimensional
  • Electrophoresis, Polyacrylamide Gel
  • Hydrogen-Ion Concentration
  • Kinetics
  • Mass Spectrometry
  • Peptidoglycan
  • Plankton / metabolism
  • Polymers / chemistry
  • Principal Component Analysis
  • Protein Structure, Tertiary
  • Proteome
  • Proteomics / methods*
  • Silver Staining
  • Time Factors
  • Yersinia / metabolism*

Substances

  • Bacterial Outer Membrane Proteins
  • Bacterial Proteins
  • Peptidoglycan
  • Polymers
  • Proteome