The selenium status and the relationship of whole-blood selenium and plasma homocysteine are reported for healthy human subjects living in Upper Silesia. A total of 1063 individuals (627 male and 436 female) examined for whole-blood selenium were subdivided into six groups according to age; the youngest included adolescents (n=143) aged 10-15 yr, and the oldest were centenarians (n=132). The mean Se content was relatively low (62.5+/-18.4 microg/L), and it tended to be higher in men (65.9+/-17.2 microg/L) than in women (57.5+/-18.9 microg/L). Selenium levels appeared to be age dependent, as the highest values were observed in young and middle-age adults (21-40 yr), whereas they were significantly lower in adolescents and in the elderly. In more than 40% of apparently healthy adults (aged 21-69 yr), the Se concentration was within the range 60-80 microg/L (i.e., below the lower limit of the nutritional adequacy range [80 microg/L]). A significant inverse correlation between whole-blood selenium and plasma total homocysteine was detected in a smaller population sample of middle-aged and elderly persons (n=204).