Quantitation of Bacillus clausii in biological samples by real-time polymerase chain reaction

J Microbiol Methods. 2006 Jun;65(3):632-6. doi: 10.1016/j.mimet.2005.10.010. Epub 2005 Nov 28.

Abstract

A real-time PCR assay targeting the highly specific erm34 sequence of Bacillus clausii DNA was developed and optimized. The quantitative assay showed a sensitivity level of 10(2) CFU/microl of sample. The method may represent a useful tool for monitoring the role of B. clausii as probiotic in vivo.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • Aztreonam / pharmacology
  • Bacillus / genetics
  • Bacillus / growth & development*
  • Bacillus / isolation & purification*
  • Bacterial Proteins / genetics
  • Chloramphenicol / pharmacology
  • Colony Count, Microbial
  • Culture Media
  • DNA, Bacterial / analysis
  • DNA, Bacterial / isolation & purification
  • Drug Resistance, Bacterial
  • Feces / microbiology*
  • Humans
  • Methyltransferases / genetics*
  • Polymerase Chain Reaction / methods*
  • Probiotics

Substances

  • Anti-Bacterial Agents
  • Bacterial Proteins
  • Culture Media
  • DNA, Bacterial
  • Chloramphenicol
  • Methyltransferases
  • Aztreonam