Methyl oleate modulates LIP2 expression in the lipolytic yeast Yarrowia lipolytica

Biotechnol Lett. 2005 Nov;27(22):1751-4. doi: 10.1007/s10529-005-3547-2.

Abstract

Methyl oleate was used as a primary carbon source and as an alternative inducer for the production of an extracellular lipase, Lip2, in Y. lipolytica strain LgX64.81 grown in a 20-l bioreactor. The lipase-encoding gene, LIP2, was investigated during culture on methyl oleate using a pLIP2-LacZ reporter fusion and we provide evidence for the involvement of methyl oleate in its regulation.

MeSH terms

  • Bacterial Proteins / biosynthesis*
  • Cell Culture Techniques / methods
  • Cell Proliferation / drug effects
  • Dose-Response Relationship, Drug
  • Fungal Proteins
  • Gene Expression Regulation, Enzymologic / drug effects
  • Gene Expression Regulation, Enzymologic / physiology*
  • Gene Expression Regulation, Fungal / drug effects
  • Gene Expression Regulation, Fungal / physiology*
  • Lipase / biosynthesis*
  • Lipolysis / drug effects
  • Lipolysis / physiology*
  • Oleic Acids / administration & dosage*
  • Yarrowia / drug effects
  • Yarrowia / physiology*

Substances

  • Bacterial Proteins
  • Fungal Proteins
  • Oleic Acids
  • methyl oleate
  • LIP2 protein, Yarrowia lipolytica
  • Lipase