Abstract
Methyl oleate was used as a primary carbon source and as an alternative inducer for the production of an extracellular lipase, Lip2, in Y. lipolytica strain LgX64.81 grown in a 20-l bioreactor. The lipase-encoding gene, LIP2, was investigated during culture on methyl oleate using a pLIP2-LacZ reporter fusion and we provide evidence for the involvement of methyl oleate in its regulation.
MeSH terms
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Bacterial Proteins / biosynthesis*
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Cell Culture Techniques / methods
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Cell Proliferation / drug effects
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Dose-Response Relationship, Drug
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Fungal Proteins
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Gene Expression Regulation, Enzymologic / drug effects
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Gene Expression Regulation, Enzymologic / physiology*
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Gene Expression Regulation, Fungal / drug effects
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Gene Expression Regulation, Fungal / physiology*
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Lipase / biosynthesis*
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Lipolysis / drug effects
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Lipolysis / physiology*
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Oleic Acids / administration & dosage*
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Yarrowia / drug effects
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Yarrowia / physiology*
Substances
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Bacterial Proteins
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Fungal Proteins
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Oleic Acids
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methyl oleate
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LIP2 protein, Yarrowia lipolytica
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Lipase