Many studies support the supposition that HSPs expressed on the cell membrane play an important role in cancer immunity. In the present study, we demonstrated that HSP60 and HSP70 are markedly increased on the cell membrane of human Epstein-Barr virus (EBV) transformed B cells. In order to investigate whether these molecules were involved in the response of human gammasigma T cells to transformed cells, the cytotoxicities of gammasigma T cells to transformed cells with or without an HSP60/70 gene knockdown were evaluated. gammasigma T cells showed marked cytotoxities to transformed cells. Down-regulation of HSP70 expression could inhibit the reactions, whereas down-regulation of HSP60 expression had little such effect. Moreover, HSP72 could significantly induce human gammasigma T cells to proliferate in vitro. Taken together, our data indicated that HSP60 and HSP70 could be valuable biomarkers for the prediction of early stage in tumorigenesis. Additionally, HSP72 might be a potential candidate of the adjuvant for gammasigma T cells in tumor immunotherapy.