Abstract
The DNA aggregation was found in aloe-emodin-induced H460 cell apoptosis in this study. Aloe-emodin (40microM)-induced DNA single strand breaks were observed by comet assay. Aloe-emodin induced decreases in the mRNA of DNA repair enzymes such as hMTH1, hOGG1 and APE. Although the activity of the radical-scavenging enzyme SOD was enhanced by aloe-emodin, the effects of aloe-emodin on H460 cell apoptosis were suspected to result from the prooxidant. These results suggest that aloe-emodin induced DNA damage through generation of reactive oxygen species in human lung carcinoma cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anthraquinones
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Apoptosis / drug effects
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Comet Assay
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DNA Damage / drug effects*
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DNA Glycosylases / genetics
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DNA Glycosylases / metabolism
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DNA Repair / drug effects
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DNA Repair Enzymes / genetics
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DNA Repair Enzymes / metabolism
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DNA, Neoplasm / drug effects*
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DNA-(Apurinic or Apyrimidinic Site) Lyase / genetics
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DNA-(Apurinic or Apyrimidinic Site) Lyase / metabolism
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Emodin / therapeutic use*
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Enzyme Inhibitors / therapeutic use*
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Humans
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Lung Neoplasms / drug therapy*
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Lung Neoplasms / metabolism
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Phosphoric Monoester Hydrolases / genetics
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Phosphoric Monoester Hydrolases / metabolism
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Reactive Oxygen Species / metabolism*
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Reverse Transcriptase Polymerase Chain Reaction
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Tumor Cells, Cultured
Substances
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Anthraquinones
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DNA, Neoplasm
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Enzyme Inhibitors
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RNA, Messenger
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Reactive Oxygen Species
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aloe emodin
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Phosphoric Monoester Hydrolases
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DNA Glycosylases
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oxoguanine glycosylase 1, human
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8-oxodGTPase
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APEX1 protein, human
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DNA-(Apurinic or Apyrimidinic Site) Lyase
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DNA Repair Enzymes
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Emodin