Toll-like receptor 2 deficiency delays pneumococcal phagocytosis and impairs oxidative killing by granulocytes

Maryse Letiembre; Hakim Echchannaoui; Philipp Bachmann; Fabrizia Ferracin; Concepción Nieto; Manuel Espinosa; Regine Landmann

doi:10.1128/IAI.73.12.8397-8401.2005

Toll-like receptor 2 deficiency delays pneumococcal phagocytosis and impairs oxidative killing by granulocytes

Infect Immun. 2005 Dec;73(12):8397-401. doi: 10.1128/IAI.73.12.8397-8401.2005.

Authors

Maryse Letiembre¹, Hakim Echchannaoui, Philipp Bachmann, Fabrizia Ferracin, Concepción Nieto, Manuel Espinosa, Regine Landmann

Affiliation

¹ Division of Infectious Diseases, Department of Research, University Hospital, Hebelstrasse 20, CH-4031 Basel, Switzerland.

Abstract

Phagocytosis and killing of Streptococcus pneumoniae was compared in blood-derived wild-type (WT) and Toll-like receptor 2 (TLR2)-deficient (TLR2-/-) polymorphonuclear leukocytes (PMN). Phagocytosis of green fluorescent protein-transformed pneumococci was delayed in TLR2-/- PMN. These cells exhibited also a lower oxidative bactericidal activity against S. pneumoniae than WT PMN, suggesting that TLR2 modulates bacterial clearance in PMN.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cells, Cultured
Granulocytes / chemistry
Granulocytes / immunology
Granulocytes / microbiology*
Green Fluorescent Proteins / analysis
Mice
Mice, Knockout
Oxidation-Reduction
Phagocytosis* / genetics
Streptococcus pneumoniae / immunology*
Toll-Like Receptor 2 / deficiency*
Toll-Like Receptor 2 / genetics

Substances

Toll-Like Receptor 2
Green Fluorescent Proteins