Identification and phylogenetic analysis of Arsenophonus- and Photorhabdus-type bacteria from adult Hippoboscidae and Streblidae (Hippoboscoidea)

J Invertebr Pathol. 2006 Jan;91(1):64-8. doi: 10.1016/j.jip.2005.08.009. Epub 2005 Nov 10.

Abstract

This is the first report of Arsenophonus- and Photorhabdus-type bacteria from Streblidae (bat flies) and Hippoboscidae (louse flies, keds). Strains were detected by means of polymerase chain reaction of 16S rDNA, and phylogenetic analysis determined the relationship of the obtained sequences to previously reported sequences in GenBank. Phylogenetic analysis by means of maximum parsimony revealed that all isolated Arsenophonus spp. 16S rDNA sequences formed a monophyletic sub-clade within other insect Arsenophonus spp., while the Photorhabdus spp. sequences are part of a monophyletic clade including Photorhabdus spp., Xenorhabdus spp. and Proteus spp.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Diptera / microbiology*
  • Enterobacteriaceae / genetics*
  • Molecular Sequence Data
  • Phylogeny*
  • Polymerase Chain Reaction
  • RNA, Bacterial / analysis
  • RNA, Bacterial / genetics*
  • RNA, Ribosomal, 16S / genetics
  • Sequence Homology

Substances

  • RNA, Bacterial
  • RNA, Ribosomal, 16S