Abstract
Eight anaerobic granular sludges were surveyed for Crenarchaeota using rRNA gene cloning. Microbial arrangement and substrate uptake patterns were elucidated by fluorescent in situ hybridization and beta imaging. Group 1.3 Crenarchaeota represented up to 50% of Archaea and 25% of the total microbiota in five sludges. Crenarchaeota were localized in close association with methanogenic Archaea.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anaerobiosis
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Crenarchaeota / genetics*
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Crenarchaeota / growth & development
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Crenarchaeota / isolation & purification*
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Crenarchaeota / metabolism
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DNA, Archaeal / analysis
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DNA, Ribosomal / analysis
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Genes, rRNA
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In Situ Hybridization, Fluorescence
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Molecular Sequence Data
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Phylogeny*
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RNA, Ribosomal, 16S / genetics
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Sewage / microbiology*
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Waste Disposal, Fluid / methods*
Substances
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DNA, Archaeal
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DNA, Ribosomal
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RNA, Ribosomal, 16S
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Sewage