Abstract
A new type of fluorescent nucleic acid probes, 2-bis-pyrene-modified oligonucleotides, is described. Preparation of these conjugates involves attachment of two pyrene moieties to the 2'-phosphate group introduced into any position within a sequence by solid-phase phosphoramidite synthesis. Good hybridization properties of the 2'-bis-pyrene probes, their nuclease resistance and sensitivity of fluorescence to the type of complementary nucleic acid have been demonstrated.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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DNA / chemistry
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Fluorescence Resonance Energy Transfer
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Fluorescent Dyes / pharmacology*
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Hydrogen-Ion Concentration
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Models, Chemical
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Molecular Biology / methods
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Nucleic Acid Hybridization
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Nucleic Acids / chemistry*
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Oligonucleotide Probes / chemistry*
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Oligonucleotides / chemistry*
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Phosphates / chemistry
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Pyrenes / chemistry
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RNA / chemistry
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Spectrometry, Fluorescence
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Temperature
Substances
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Fluorescent Dyes
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Nucleic Acids
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Oligonucleotide Probes
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Oligonucleotides
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Phosphates
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Pyrenes
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RNA
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DNA