2'-bis-pyrene modified oligonucleotides: sensitive fluorescent probes of nucleic acids structure

Nucleosides Nucleotides Nucleic Acids. 2005;24(5-7):729-34. doi: 10.1081/ncn-200060038.

Abstract

A new type of fluorescent nucleic acid probes, 2-bis-pyrene-modified oligonucleotides, is described. Preparation of these conjugates involves attachment of two pyrene moieties to the 2'-phosphate group introduced into any position within a sequence by solid-phase phosphoramidite synthesis. Good hybridization properties of the 2'-bis-pyrene probes, their nuclease resistance and sensitivity of fluorescence to the type of complementary nucleic acid have been demonstrated.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA / chemistry
  • Fluorescence Resonance Energy Transfer
  • Fluorescent Dyes / pharmacology*
  • Hydrogen-Ion Concentration
  • Models, Chemical
  • Molecular Biology / methods
  • Nucleic Acid Hybridization
  • Nucleic Acids / chemistry*
  • Oligonucleotide Probes / chemistry*
  • Oligonucleotides / chemistry*
  • Phosphates / chemistry
  • Pyrenes / chemistry
  • RNA / chemistry
  • Spectrometry, Fluorescence
  • Temperature

Substances

  • Fluorescent Dyes
  • Nucleic Acids
  • Oligonucleotide Probes
  • Oligonucleotides
  • Phosphates
  • Pyrenes
  • RNA
  • DNA