3'-modified oligo (2'-O-methylribonucleotides) as improved probes for hybridization with RNA

Nucleosides Nucleotides Nucleic Acids. 2005;24(5-7):527-31. doi: 10.1081/ncn-200061795.

Abstract

A series of octa (2-O-methylribonucleotides) with an additional 3'-terminal deoxynucleoside (T, dC, dA or dG) linked by the 3'-3' (inverted) bond was synthesized. The exceptional stability of these oligomers to a 3'-exonuclease (SVP) and nucleases in culture medium containing 10% heat-inactivated fetal calf serum was demonstrated. It was shown that the addition of the 3'-dangling inverted deoxynucleoside increases substantially the thermal stability of the duplexes of oligo(2'-O-methylribonucleotides) with complementary RNA and DNA in the case of a relatively weak terminal AmU(T) pair and enhances the mismatch sensitivity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Pair Mismatch
  • Base Sequence
  • DNA / chemistry
  • DNA, Complementary / metabolism
  • Deoxyribonucleotides / chemistry
  • Kinetics
  • Models, Chemical
  • Molecular Sequence Data
  • Nucleic Acid Conformation
  • Nucleic Acid Heteroduplexes
  • Nucleic Acid Hybridization
  • Oligonucleotides / chemistry
  • Oligoribonucleotides / chemistry
  • RNA / chemistry*
  • RNA, Complementary / chemistry
  • Ribonucleotides / chemistry*
  • Temperature
  • Thermodynamics

Substances

  • DNA, Complementary
  • Deoxyribonucleotides
  • Nucleic Acid Heteroduplexes
  • Oligonucleotides
  • Oligoribonucleotides
  • RNA, Complementary
  • Ribonucleotides
  • RNA
  • DNA