Abstract
A series of octa (2-O-methylribonucleotides) with an additional 3'-terminal deoxynucleoside (T, dC, dA or dG) linked by the 3'-3' (inverted) bond was synthesized. The exceptional stability of these oligomers to a 3'-exonuclease (SVP) and nucleases in culture medium containing 10% heat-inactivated fetal calf serum was demonstrated. It was shown that the addition of the 3'-dangling inverted deoxynucleoside increases substantially the thermal stability of the duplexes of oligo(2'-O-methylribonucleotides) with complementary RNA and DNA in the case of a relatively weak terminal AmU(T) pair and enhances the mismatch sensitivity.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Pair Mismatch
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Base Sequence
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DNA / chemistry
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DNA, Complementary / metabolism
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Deoxyribonucleotides / chemistry
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Kinetics
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Models, Chemical
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Molecular Sequence Data
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Nucleic Acid Conformation
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Nucleic Acid Heteroduplexes
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Nucleic Acid Hybridization
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Oligonucleotides / chemistry
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Oligoribonucleotides / chemistry
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RNA / chemistry*
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RNA, Complementary / chemistry
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Ribonucleotides / chemistry*
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Temperature
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Thermodynamics
Substances
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DNA, Complementary
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Deoxyribonucleotides
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Nucleic Acid Heteroduplexes
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Oligonucleotides
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Oligoribonucleotides
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RNA, Complementary
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Ribonucleotides
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RNA
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DNA