Abstract
Artepillin C, a prenylated phenylpropanoid found specifically in Brazilian propolis, has been shown to be a bioavailable antioxidant. In this study, artepillin C was tested for colon cancer-preventing activity using azoxymethane-challenged ddY mice. Oral doses of 80 and 160 mg/kg body weight of propolis or 10mg/kg of artepillin C (equi-amounts to 160 mg propolis) reduced significantly the frequency of colonic aberrant crypt foci (ACF) by 39.2, 43.7 and 43.4%, respectively. In liver of the mice, glutathione S-transferase and NADPH:quinone reductase activity increased with the doses of propolis or artepillin C, and an antioxidant-responsive element (ARE) was found to be activated for binding DNA. Artepillin C is considered to suppress the formation of colonic ACF through the activation of ARE and induction of phase II enzymes in liver.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antineoplastic Agents / administration & dosage
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Antineoplastic Agents / pharmacology*
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Antioxidants / pharmacology*
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Azoxymethane
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Carcinogens
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Colonic Neoplasms / chemically induced
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Colonic Neoplasms / prevention & control*
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Diet
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Dose-Response Relationship, Drug
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Glutathione Transferase / genetics
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Glutathione Transferase / metabolism
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Intestinal Mucosa / drug effects*
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Intestinal Mucosa / pathology
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Liver / drug effects
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Liver / enzymology
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Male
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Mice
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NAD(P)H Dehydrogenase (Quinone) / genetics
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NAD(P)H Dehydrogenase (Quinone) / metabolism
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Phenylpropionates / administration & dosage
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Phenylpropionates / pharmacology*
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Precancerous Conditions / chemically induced
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Precancerous Conditions / prevention & control*
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Propolis / administration & dosage
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Propolis / pharmacology
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Response Elements / drug effects
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Up-Regulation
Substances
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Antineoplastic Agents
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Antioxidants
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Carcinogens
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Phenylpropionates
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artepillin C
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Propolis
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NAD(P)H Dehydrogenase (Quinone)
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Glutathione Transferase
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Azoxymethane