Time-course data analysis of gene expression profiles reveals purR regulon concerns in organic solvent tolerance in Escherichia coli

Kazunori Shimizu; Shuhei Hayashi; Noriyuki Doukyu; Takeshi Kobayashi; Hiroyuki Honda

doi:10.1263/jbb.99.72

Time-course data analysis of gene expression profiles reveals purR regulon concerns in organic solvent tolerance in Escherichia coli

J Biosci Bioeng. 2005 Jan;99(1):72-4. doi: 10.1263/jbb.99.72.

Authors

Kazunori Shimizu¹, Shuhei Hayashi, Noriyuki Doukyu, Takeshi Kobayashi, Hiroyuki Honda

Affiliation

¹ Department of Biotechnology, School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.

PMID: 16233757
DOI: 10.1263/jbb.99.72

Abstract

A time-course gene-expression profile was generated for Escherichia coli TK31 when it was exposed to an organic solvent mixture, and classified by fuzzy adaptive resonance theory (Fuzzy ART). It was found that the purR regulon plays an important role in the organic solvent tolerance (OST) of E. coli.

MeSH terms

Drug Tolerance / physiology*
Escherichia coli / drug effects
Escherichia coli / genetics
Escherichia coli / metabolism*
Escherichia coli Proteins / genetics
Escherichia coli Proteins / metabolism*
Fuzzy Logic
Gene Expression Profiling / methods*
Gene Expression Regulation, Bacterial / drug effects
Gene Expression Regulation, Bacterial / physiology
Metabolic Clearance Rate
Oligonucleotide Array Sequence Analysis / methods
Organic Chemicals / pharmacology*
Regulon / genetics
Repressor Proteins / genetics
Repressor Proteins / metabolism*
Solvents / pharmacology*

Substances

Escherichia coli Proteins
Organic Chemicals
PurR protein, E coli
Repressor Proteins
Solvents