Novel fluorogenic substrates for imaging beta-lactamase gene expression

J Am Chem Soc. 2003 Sep 17;125(37):11146-7. doi: 10.1021/ja036126o.

Abstract

A new class of small nonfluorescent fluorogenic substrates becomes brightly fluorescent after beta-lactamase hydrolysis with up to 153-fold enhancement in the fluorescence intensity. Less than 500 fM of beta-lactamase in cell lysates can be readily detected, and beta-lactamase expression in living cells can be imaged with a red fluorescence derivative. These new fluorogenic substrates should find uses in clinical diagnostics and facilitate the applications of beta-lactamase as a biosensor.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cell Line
  • Fluorescent Dyes / analysis*
  • Fluorescent Dyes / chemistry*
  • Gene Expression
  • Humans
  • Hydrolysis
  • Linear Models
  • Molecular Structure
  • beta-Lactamases / analysis*
  • beta-Lactamases / chemistry
  • beta-Lactamases / metabolism*

Substances

  • Fluorescent Dyes
  • beta-Lactamases