Abstract
We designed a multiplex PCR for the detection of all categories of diarrheagenic Escherichia coli. This method proved to be specific and rapid in detecting virulence genes from Shiga toxin-producing (stx(1), stx(2), and eae), enteropathogenic (eae and bfp), enterotoxigenic (st II and lt), enteroinvasive (vir F and ipa H), entero-aggregative (aaf II), and diffuse adherent (daa E) Escherichia coli in stool samples.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Toxins / genetics
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Bacterial Toxins / metabolism
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Child
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DNA, Bacterial / analysis
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Diarrhea / microbiology*
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Enterotoxins / genetics
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Enterotoxins / metabolism
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Escherichia coli / classification*
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Escherichia coli / genetics
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Escherichia coli / pathogenicity
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Escherichia coli Infections / microbiology*
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Escherichia coli Proteins / genetics
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Escherichia coli Proteins / metabolism
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Humans
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Polymerase Chain Reaction / methods*
Substances
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Bacterial Toxins
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DNA, Bacterial
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Enterotoxins
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Escherichia coli Proteins