Single multiplex PCR assay to identify simultaneously the six categories of diarrheagenic Escherichia coli associated with enteric infections

J Clin Microbiol. 2005 Oct;43(10):5362-5. doi: 10.1128/JCM.43.10.5362-5365.2005.

Abstract

We designed a multiplex PCR for the detection of all categories of diarrheagenic Escherichia coli. This method proved to be specific and rapid in detecting virulence genes from Shiga toxin-producing (stx(1), stx(2), and eae), enteropathogenic (eae and bfp), enterotoxigenic (st II and lt), enteroinvasive (vir F and ipa H), entero-aggregative (aaf II), and diffuse adherent (daa E) Escherichia coli in stool samples.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Toxins / genetics
  • Bacterial Toxins / metabolism
  • Child
  • DNA, Bacterial / analysis
  • Diarrhea / microbiology*
  • Enterotoxins / genetics
  • Enterotoxins / metabolism
  • Escherichia coli / classification*
  • Escherichia coli / genetics
  • Escherichia coli / pathogenicity
  • Escherichia coli Infections / microbiology*
  • Escherichia coli Proteins / genetics
  • Escherichia coli Proteins / metabolism
  • Humans
  • Polymerase Chain Reaction / methods*

Substances

  • Bacterial Toxins
  • DNA, Bacterial
  • Enterotoxins
  • Escherichia coli Proteins