Use of stored serum from Uganda for development and evaluation of a human immunodeficiency virus type 1 testing algorithm involving multiple rapid immunoassays

Darrell E Singer; Noah Kiwanuka; David Serwadda; Fred Nalugoda; Linda Hird; Jamie Bulken-Hoover; Godfrey Kigozi; Jennifer A Malia; Eva K Calero; Warren Sateren; Merlin L Robb; Fred Wabwire-Mangen; Maria Wawer; Ronald H Gray; Nelson Sewankambo; Deborah L Birx; Nelson L Michael

doi:10.1128/JCM.43.10.5312-5315.2005

Use of stored serum from Uganda for development and evaluation of a human immunodeficiency virus type 1 testing algorithm involving multiple rapid immunoassays

J Clin Microbiol. 2005 Oct;43(10):5312-5. doi: 10.1128/JCM.43.10.5312-5315.2005.

Authors

Darrell E Singer¹, Noah Kiwanuka, David Serwadda, Fred Nalugoda, Linda Hird, Jamie Bulken-Hoover, Godfrey Kigozi, Jennifer A Malia, Eva K Calero, Warren Sateren, Merlin L Robb, Fred Wabwire-Mangen, Maria Wawer, Ronald H Gray, Nelson Sewankambo, Deborah L Birx, Nelson L Michael

Affiliation

¹ Division of Retrovirology, WalterReed Army Institute of Research, Rockville, MD 20850, USA.

Abstract

We report the development and evaluation of a human immunodeficiency virus type 1 testing algorithm consisting of three rapid antibody detection tests. Stored serum samples from Uganda were utilized with a final algorithm sensitivity of 100% and a specificity of 98.9% (95% confidence interval, 98.6% to 99.3%).

Publication types

Evaluation Study
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

AIDS Serodiagnosis*
Algorithms*
Confidence Intervals
HIV Antibodies / blood*
HIV Infections / diagnosis*
HIV Infections / virology
HIV-1 / immunology*
Humans
Immunoassay / methods
Sensitivity and Specificity
Serum
Specimen Handling / methods*
Time Factors
Uganda

Substances

HIV Antibodies