Abstract
In our investigation of in vitro anti-allergic screening of medicinal herbal extracts, the ethyl acetate extract of the root of Wikstroemia indica was observed to inhibit nitric oxide (NO) production in a lipopolysaccharide (LPS) and recombinant mouse interferon-gamma (IFN-gamma) activated murine macrophage-like cell line, RAW 264.7. Fractionation of the active extract led to the isolation of two new lignan dimer bis-5,5-nortrachelogenin (1) and bis-5,5'-nortrachelogenin (2), and three known lignans. Compound 1 and lirioresinol B (5) inhibited NO production with IC50 values at 48.6 and 53.5 microM, respectively. Compound 5 also showed free radical scavenging effect on 1,1-diphenyl-2-picrylhydrazil (DPPH) at IC50 values at 19.5 microM.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Biphenyl Compounds
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Carbon Isotopes
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Cell Line
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Drug Evaluation, Preclinical
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Free Radical Scavengers / chemistry
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Free Radical Scavengers / isolation & purification
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Free Radical Scavengers / pharmacology*
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Interferon-gamma / antagonists & inhibitors
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Lignans / chemistry
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Lignans / isolation & purification
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Lignans / pharmacology*
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Lipopolysaccharides
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Macrophages / drug effects
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Magnetic Resonance Spectroscopy / methods
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Magnetic Resonance Spectroscopy / standards
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Mice
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Molecular Structure
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Nitric Oxide / antagonists & inhibitors*
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Nitric Oxide / biosynthesis
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Picrates / chemistry*
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Plant Extracts / chemistry
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Plant Extracts / isolation & purification
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Plant Extracts / pharmacology
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Plant Roots / chemistry
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Protons
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Recombinant Proteins / drug effects
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Reference Standards
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Wikstroemia / chemistry*
Substances
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Biphenyl Compounds
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Carbon Isotopes
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Free Radical Scavengers
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Lignans
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Lipopolysaccharides
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Picrates
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Plant Extracts
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Protons
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Recombinant Proteins
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Nitric Oxide
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Interferon-gamma
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1,1-diphenyl-2-picrylhydrazyl