Objective: To isolate hantavirus and to genotype isolated strains of hantavirus (SY 13) in Liaoning province.
Methods: Hantavirus was isolated by means of cell culture. The G1, G2 fragments of M segments and S segment of the strain of SY 13 were amplified respectively by means of RT-PCR in order to compare and analyze the homology between the sequences of the three isolated gene fragments and the sequences downloaded from GenBank with neighbor-joining through sequence analysis software of DNA Star, Clustal, Phylip, etc.
Results: SY 13 and HTN showed higher homology, reached up to 79.3%-97.0%; SY 13 and BAO 10, BAO 14, JIANG 13, BAO 9, Q 33 were on the same branch and their homology reached up to 95.0%-97.0%.
Conclusion: The isolated hantavirus strain in Liaoning area was the same as the one in Heilongjiang area which belongs to HTN H4.