[Molecular characterization of a glycopeptide-resistant Enterococcus faecalis outbreak in an intensive care unit]

Enferm Infecc Microbiol Clin. 2005 Oct;23(8):460-3. doi: 10.1157/13078822.
[Article in Spanish]

Abstract

Introduction: Between January and August 2003 glycopeptide-resistant Enterococcus faecalis was isolated from eight patients admitted to the intensive care unit (ICU).

Methods: Antibiotic susceptibility testing was performed by disk diffusion and the Etest, clonal relatedness of the isolates was studied by pulsed-field gel electrophoresis (PFGE), and the presence of vanA was investigated by PCR.

Results and conclusions: All the isolates were vanA-positive and had an identical PFGE pattern, showing that an outbreak had occurred in our ICU.

Publication types

  • English Abstract

MeSH terms

  • Bacterial Proteins / genetics
  • Carbon-Oxygen Ligases / genetics
  • Cross Infection / drug therapy
  • Cross Infection / microbiology*
  • Electrophoresis, Gel, Pulsed-Field
  • Enterococcus faecalis / drug effects*
  • Enterococcus faecalis / genetics
  • Gram-Positive Bacterial Infections / drug therapy
  • Gram-Positive Bacterial Infections / microbiology*
  • Humans
  • Intensive Care Units
  • Polymerase Chain Reaction
  • Spain
  • Vancomycin Resistance*

Substances

  • Bacterial Proteins
  • VanA ligase, Bacteria
  • Carbon-Oxygen Ligases