Abstract
Reversed-phase preparative HPLC analysis of the methanol extract of the seeds of Ilex aquifolium afforded two antioxidant phenylacetic acid derivatives, 2,4-dihydroxyphenylacetic acid (1) and 2,4-dihydroxyphenylacetic acid methyl ester (2). The structures were determined by spectroscopic methods. In the DPPH assay for antioxidant activity, the IC(50) values of 1 and 2 were 1.50 x 10(-3) and 2.55 x 10(-3) mg mL(-1), respectively, compared to 2.88 x 10(-5) mg mL(-1) of quercetin, a natural antioxidant.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antioxidants / chemistry*
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Antioxidants / isolation & purification
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Biphenyl Compounds / chemistry
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Chromatography, High Pressure Liquid
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Chromatography, Thin Layer
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Hydrazines / chemistry
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Ilex / chemistry*
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Magnetic Resonance Spectroscopy
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Phenylacetates / chemistry*
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Phenylacetates / isolation & purification
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Picrates
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Seeds / chemistry
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Spectrophotometry, Ultraviolet
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Stereoisomerism
Substances
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Antioxidants
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Biphenyl Compounds
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Hydrazines
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Phenylacetates
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Picrates
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1,1-diphenyl-2-picrylhydrazyl