Abstract
Type B leukemogenic virus is a variant of mouse mammary tumor virus (MMTV) that causes thymic lymphomas rather than mammary tumors in mice. We demonstrate that conversion of a mammotropic MMTV to a T-cell-tropic virus requires two alterations in the long terminal repeat: (i) acquisition of a T-cell-specific enhancer and (ii) loss of transcriptional repression through deletion of negative regulatory elements (NREs) or by suppression of NRE activity after appropriate positioning of the enhancer.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Cell Line
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Enhancer Elements, Genetic
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Genes, Regulator*
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Humans
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Jurkat Cells
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Lymphoma, T-Cell / virology
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Mammary Neoplasms, Experimental / virology
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Mammary Tumor Virus, Mouse / genetics*
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Mammary Tumor Virus, Mouse / pathogenicity*
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Mice
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Mice, Inbred BALB C
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Proviruses / genetics
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Proviruses / pathogenicity
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Retroviridae Infections / virology
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T-Lymphocytes / virology
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Terminal Repeat Sequences*
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Transcription, Genetic
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Transfection
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Tumor Virus Infections / virology