Allosteric modulation of the nicotinic acetylcholine receptor by physostigmine

Ann N Y Acad Sci. 2005 Jun:1048:355-8. doi: 10.1196/annals.1342.037.

Abstract

The action of physostigmine on mouse muscle nicotinic acetylcholine receptor expressed in the COS-7 cell line was studied by the patch-clamp technique. Physostigmine accelerated, by allosteric modulation, the rate of desensitization of whole cell currents induced by acetylcholine and decreased the maximal amplitude in concentration-dependent manner.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Allosteric Regulation / drug effects*
  • Animals
  • COS Cells
  • Chlorocebus aethiops
  • Cholinesterase Inhibitors / pharmacology*
  • Dose-Response Relationship, Drug
  • Neurons / drug effects*
  • Neurons / physiology
  • Patch-Clamp Techniques
  • Physostigmine / pharmacology*
  • Receptors, Nicotinic / metabolism*
  • Time Factors

Substances

  • Cholinesterase Inhibitors
  • Receptors, Nicotinic
  • Physostigmine