Imidazoline ring system is the structural element of many drugs which have different pharmacological spectrum of activity as ligands of the imidazoline receptor. Besides, from the literature data it follows that depending on the type of substituent certain derivatives of imidazoline may also show antimicrobial properties. The obtained compounds were tested for their potential antimicrobial activity. Microbiological tests conducted on 106 strains of bacteria, 6 strains of yeast-like fungi and 3 strains of moulds have shown that all the tested compounds in the examined concentrations (100 mg ml(-1) and 200 mg ml(-1)) had no influence on the growth of tested bacteria, yeast-like fungi and moulds.