1,2,4-Triazole is the structural element of many drugs that have different pharmacological spectrum of activity. Besides, from the literature data it follows that 1,2,4-triazoles and its fused systems show antibacterial, antifungal and anti-inflammatory activities. The obtained compounds were tested for their potential antimicrobial activity. Microbiological tests conducted on 106 strains of bacteria, 6 strains of yeast-like fungi and 3 strains of moulds have shown that all the tested compounds in the examined concentrations (100 microg ml(-1) and 200 microg ml(-1)) had no influence on the growth of the tested bacteria and moulds. It has been found that among variously substituted derivatives of imidazotriazole system compound V significantly inhibited the growth of 6 strains of Candida albicans in the examined concentrations. Its chemical structure was similar to novel antifungal agents described in the literature.