Abstract
Intracellular dialysis and membrane voltage clamping were used to show that He-Ne laser irradiation of a pond snail neuron at a dose of 0.7 x 10(-4) J (power density 1.5 x 10(2) W/m2) increases the amplitude of the potential-dependent slow potassium current, while a dose of 0.7 x 10(-3) J decreases this current. Bupivacaine suppresses the potassium current. Combined application of laser irradiation at a dose of 0.7 x 10(-3) J increased the blocking effect of 10 microM bupivacaine on the slow potassium current, while an irradiation dose of 0.7 x 10(-4) J weakened the effect of bupivacaine.
MeSH terms
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Anesthetics, Local / pharmacology
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Animals
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Bupivacaine / pharmacology
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Ganglia, Invertebrate / cytology
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Ganglia, Invertebrate / drug effects
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Ganglia, Invertebrate / radiation effects
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Helium
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Lasers*
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Low-Level Light Therapy
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Lymnaea / cytology
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Lymnaea / drug effects
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Lymnaea / physiology*
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Lymnaea / radiation effects
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Membrane Potentials / drug effects
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Membrane Potentials / physiology*
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Membrane Potentials / radiation effects
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Neon
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Neurons / drug effects
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Neurons / physiology*
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Neurons / radiation effects
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Patch-Clamp Techniques
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Potassium Channels / drug effects
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Potassium Channels / physiology*
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Potassium Channels / radiation effects
Substances
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Anesthetics, Local
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Potassium Channels
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Helium
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Neon
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Bupivacaine