Objective: To observe the hematopoietic effects of Siwu decoction in the mice with blood deficiency induced by compound method of bleeding, starved feeding and exhausting and lay experimental basis under the blood-enriching function of Siwu decoction in different models of blood deficiency.
Method: The animal model of blood deficiency was established by a combination of starved feeding with routine food at the rate of 75 g(-1) kg(-1) body weight per day, swimming 20 minutes and removing 0.4 mL blood every other day in each mouse. The amount of peripheral blood cells, the cell cycle and the apoptosis of bone marrow cells and the percentage of CD34+ in bone marrow cells were detected by microcellcounter and flow cytometry.
Result: The amount of red blood cells in model mice was significantly increased by Siwu decoction at different dosages, from 4.6 x 10(12) to 6.5, 7.1 and 7.0 x 10(12) respectively. At the 15th day, the percentages of bone marrow cells at S stage in the treated group were 14.34%, 14.03% and 13.58% which were significantly higher than that in model group without treatment of Siwu Decoction.
Conclusion: Siwu decoction significantly improve the hematopoiesis in the mice with blood deficiency induced by compound method mentioned above.