Detection of Babesia microti DNA in Ixodes scapularis (Acari: Ixodidae) by use of Chelex 100 resin and polymerase chain reaction

J Med Entomol. 2005 Jul;42(4):694-6. doi: 10.1093/jmedent/42.4.694.

Abstract

A rapid and sensitive protocol using Chelex 100 resin for DNA extraction and the polymerase chain reaction for detection of Babesia microti Franca within its vector Ixodes scapularis Say is described. The level of detection of this protocol was determined to be the approximate equivalent of one parasite.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Arachnid Vectors / parasitology
  • Babesia microti / genetics*
  • Chelating Agents
  • DNA, Protozoan / analysis*
  • Ixodes / parasitology*
  • Mice
  • Polymerase Chain Reaction / methods*
  • Resins, Synthetic

Substances

  • Chelating Agents
  • DNA, Protozoan
  • Resins, Synthetic
  • Chelex 100