Detection of two functional polymorphisms in the promoter region of the IL-18 gene by single-tube allele specific PCR and melting temperature analysis

Michele Boniotto; Ludovica Segat; Michele Milanese; Sergio Crovella

doi:10.1016/j.jim.2005.06.016

Detection of two functional polymorphisms in the promoter region of the IL-18 gene by single-tube allele specific PCR and melting temperature analysis

J Immunol Methods. 2005 Sep;304(1-2):184-8. doi: 10.1016/j.jim.2005.06.016.

Authors

Michele Boniotto¹, Ludovica Segat, Michele Milanese, Sergio Crovella

Affiliation

¹ Genetics Service, IRCCS Burlo Garofolo, Via dell'Istria 65/1, 34100 Trieste, Italy.

PMID: 16111697
DOI: 10.1016/j.jim.2005.06.016

Abstract

IL-18 is an important cytokine in both innate and acquired immunity. IL-18 promoter polymorphisms have been investigated in several autoimmune and infectious diseases. Here we present two assays for a medium to a high throughput genotyping of C-607A and G-137C IL-18 SNPs. Using these protocols we have been able to rapidly genotype a cohort of 131 healthy Italian individuals. The assays, based on a multiplex allele-specific PCR and the melting temperature analysis of the PCR product, are rapid, easily automated, inexpensive and well suited to case-control association studies.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alleles*
Child
Genotype
Hot Temperature
Humans
Interleukin-18 / genetics*
Polymerase Chain Reaction / methods*
Polymorphism, Genetic*
Polymorphism, Single Nucleotide*
Promoter Regions, Genetic*

Substances

Interleukin-18