Objective: To study the effects of c9, t11-conjugated linoleic acid (c9, t11-CLA) on the critical enzyme (COX-2) and its product - prostaglandin E2 (PGE2) of linoleic acid metabolism path in human gastric adenocarcinoma cell line (SGC-7901).
Methods: Expression of COX-2 mRNA and protein were measured by reverse transcription polymerase chain reaction (RT-PCR) and Western blot, PGE2 was determined by radioimmunoassay (RIA).
Results: At the concentrations of 25, 50, 100, 200 pmol/L, c9, t11-CLA suppressed the expression of COX-2 mRNA, protein and PGE.
Conclusion: COX-2 is involved anti-cancer action of c9, t11-CLA and is likely to act as an important target.