Separation and determination of clotrimazole, methylparaben and propylparaben in pharmaceutical preparation by micellar electrokinetic chromatography

J Pharm Biomed Anal. 2006 Jan 23;40(1):215-9. doi: 10.1016/j.jpba.2005.07.001. Epub 2005 Aug 10.

Abstract

In this study, micellar electrokinetic chromatography (MEKC) method was developed for the determination of clotrimazole (CLO), methylparaben (MP) and propylparaben (PP) in a pharmaceutical preparation. Separation was carried out in a fused silica capillary (60 cm x 75 microm i.d.) at 25 kV with UV detection at 212 nm. Optimized background electrolyte (BGE) was 15 mM phosphate buffer (pH 7.2) containing 30 mM sodium dodecyl sulfate (SDS) as a surfactant. Rectilinear calibration ranges were 50-500 mg l(-1) for CLO, 10-100 mg l(-1) for MP and 2.5-25 mg l(-1) for PP. The total analysis time was < 12 min.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Infective Agents, Local / analysis
  • Anti-Infective Agents, Local / chemistry
  • Calibration
  • Chemistry Techniques, Analytical / methods*
  • Chemistry, Pharmaceutical / methods*
  • Chromatography, High Pressure Liquid
  • Chromatography, Micellar Electrokinetic Capillary / methods*
  • Clotrimazole / analysis*
  • Clotrimazole / chemistry
  • Electrolytes
  • Hydrogen-Ion Concentration
  • Models, Chemical
  • Parabens / analysis*
  • Parabens / chemistry
  • Phosphates / analysis
  • Preservatives, Pharmaceutical / analysis
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Sodium Dodecyl Sulfate / chemistry
  • Surface-Active Agents / analysis
  • Temperature
  • Time Factors

Substances

  • Anti-Infective Agents, Local
  • Electrolytes
  • Parabens
  • Phosphates
  • Preservatives, Pharmaceutical
  • Surface-Active Agents
  • Sodium Dodecyl Sulfate
  • methylparaben
  • Clotrimazole
  • propylparaben