Abstract
Inflammatory bowel disease (IBD) is characterised by oxidative and nitrosative stress, leukocyte infiltration, and up-regulation of intercellular adhesion molecule 1 (ICAM-1) expression in the colon. The aim of the present study was to examine the effects of green tea extract in rats subjected to experimental colitis induced by intracolonic instillation of dinitrobenzene sulphonic acid (DNBS). At 4 days after DNBS administration the rats were sacrificed. Treatment with green tea extract significantly attenuated diarrhoea and loss of body weight. This was associated with a remarkable amelioration of the disruption of the colonic architecture, significant reduction of colonic myeloperoxidase (MPO) and tumor necrosis factor-alpha (TNF-alpha) production. Green tea extract also reduced the appearance of nitrotyrosine immunoreactivity in the colon and reduced the up-regulation of ICAM-1.
MeSH terms
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Animals
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Benzenesulfonates
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Chromatography, High Pressure Liquid
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Colitis / chemically induced
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Colitis / drug therapy*
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Colitis / pathology
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Colitis / prevention & control
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Colon / drug effects*
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Colon / pathology
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Flavonoids / analysis
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Flavonoids / isolation & purification
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Flavonoids / therapeutic use*
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Heme Oxygenase (Decyclizing) / analysis
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Immunohistochemistry
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Intercellular Adhesion Molecule-1 / analysis
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Male
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Peroxidase / metabolism
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Phenols / analysis
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Phenols / isolation & purification
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Phenols / therapeutic use*
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Phytotherapy*
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Plant Leaves / chemistry
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Polyphenols
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Rats
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Rats, Sprague-Dawley
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Tea / chemistry*
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Tumor Necrosis Factor-alpha / metabolism
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Tyrosine / analogs & derivatives
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Tyrosine / analysis
Substances
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Benzenesulfonates
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Flavonoids
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Phenols
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Polyphenols
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Tea
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Tumor Necrosis Factor-alpha
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dinitrobenzenesulfonic acid
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Intercellular Adhesion Molecule-1
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3-nitrotyrosine
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Tyrosine
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Peroxidase
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Heme Oxygenase (Decyclizing)